Regulatory

Part:BBa_K1707013:Design

Designed by: Sylvie Lautru, Audrey Moatti   Group: iGEM15_Paris_Saclay   (2015-09-14)

cI857 under the control of RNA thermometer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the construction, we had to introduce the RNA thermometer using Gibson assembly as we saw when we sequenced this part that the cloning of K115017 had failed.


Source

This part is the result of the assembly of existing parts.

References